Cell Counting Kit-8 (CCK-8)-盟基生物科技股份有限公司

Cellular Function & Viability Assay

Cell Counting Kit-8 (CCK-8)

Cellular Function & Viability Assay

Cell Counting Kit-8 (CCK-8)

TargetMol所出品的CCK-8細胞活力檢測試劑的靈敏度優於傳統 MTT、XTT、MTS或WST-1等試劑套組。

一、產品介紹
Cell Counting Kit-8 (CCK-8) 細胞活力檢測試劑，藉由高度水溶性的 WST-8 [2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium,monosodium salt]在細胞中被脫氫酶 (dehydrogenases)還原，產生黃色產物 (formazan)，可溶於組織培養基

Cell Counting Kit-8 乃單一瓶溶液包裝且無需預先混合配方。 Cell Counting Kit-8 是非放射性的，可透過高靈敏度的比色法測定，用於確定細胞增殖和細胞毒性測定中的活細胞數量。細胞中脫氫酶活性所產生的formazan染劑的量與活細胞的數量成正比，藉此反應細胞的活力高低。

TargetMol所出品的CCK-8細胞活力檢測試劑的靈敏度優於傳統試劑: MTT、XTT、MTS或WST-1等以tetrazolium salt為基礎的試劑套組。

二、產品優點
1. 靈敏度更甚 MTT、MTS或 WST-1
2. 無細胞毒性
3. 操作簡便、無須額外多加有機溶劑
4. All-in-One一管式配方，即開即用 (Ready-to-Use)

Properties	MTT	XTT	WST-1	CCK8
Solublity of formazan	-	+	+	+
Forms	Powder	2-bottle solution	1-bottle solution	1-bottle solution
Preparation	Dissolve before use	Mix before use	Ready to use	Ready to use
Sensitivity	+	++	++	+++
Detection Speed	+	++	++	+++
Wavelerngth	560~600nM	420~480nM	420~480nM	430~490nM
Toxicity	+	-	-	-
Stability	+	-	+	++
96-well plate compatibility	+	++	++	++
Convenience	+	++	++	+++

三、產品資訊

貨號	包裝	反應數
C0005	1 mL	100 tests
	5 mL	500 tests
	10 mL	1000 tests
	30 mL	3000 tests
	100 mL	10000 tests

四、操作流程
1. 100μL cell suspension was inoculated on 96-well plate and incubated in cell incubator (37°C, 5% CO2).

2. Take the cells out of the incubator ,add 1/10 volume of Cell Counting Kit-8 (CCK-8) directly to cells in culture medium. Mix thoroughly to achieve a homogenous solution by lightly tapping the outside of the plate several times while avoiding bubbles. For 96-well plate, add 10 µl Cell Counting Kit-8 (CCK-8) per 100 µl culture medium.

3. Incubate in a cell culture incubator for 1 to 4 hours at 37°C until the color turns orange. Over incubation will give false results.

4. Place the 96-well plate on the shaking table for about 1min before the reading of the micrometer to ensure the uniform color of orifice plate.

5. The 450nm light absorption value was read by an enzyme marker and cell activity was calculated.

6. Optional: Add 10 μl of 1 % SDS (dissolve 0.1 g SDS with PBS buffer to prepare 10 ml solution) directly to 100 μl of cells to stop the reaction. Signals can be read within 3 days without affecting the absorbance values.

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