- 首頁
- 客製化服務
- Antibody discovery
- Generation
- Monoclonal antibody
Generation
Protein Expression(E. coli、HEK293 & CHO)
Recombinant antibody production
Afucosylated Antibody Expression
Knockout Plasmid & Stable Cell line Development
Knockin & Cell line Development
Point Mutation & Cell line Development
Classical method- Overexpression Cell Line
Classical method- Knockdown Cell Line
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Monoclonal antibody
一、服務介紹小鼠單株抗體具有特異性高、親和性高、遺傳穩定等優點，應用於科學研究、醫學診斷、生物醫學等領域。
Elabscience已成功為客戶完成近3000個專案服務。經過技術平臺的全面升級，在抗體的驗證和下游應用方面為客戶提供更多元的客製化服務。
二、服務優勢1. 高品質的蛋白抗原生產（同時具備原核蛋白和真核蛋白表達平臺）
2. 針對不同應用可提供40株陽性細胞株上清液供客戶篩選驗證
3. 滿足客戶各種驗證和篩選的需求，確保抗體特異性
4. 可提供親和純化的單克隆抗體3-10mg及對應融合瘤細胞株
5. 可提供WB、IHC、IF、FACS、中和功能等全面的抗體驗證服務
三、服務方案
1.流程
2.方案服務流程 到貨規格 製作週期 1. Project analysis and antigen design 1-1. Project analysis and antigen design Project analysis report 2 days 2. Protein production 2-1. Gene synthesis and Codon optimization 100µg protein sample (>80% purity)
Expression analysis report4-6 weeks 2-2. Carrier construction 2-3. Transfection and protein Expression Evaluation 2-4. Scale up protein expression and purification 2-5. SDS-PAGE analysis 3. Immunization and Titer test 3-1. Immunize 5 mice ELISA test report
(ELISA titer>1:64,000)6-9 weeks 3-2. Standard immune schedule includes 5 immunization 3-3. Indirect ELISA test 4. Hybridoma fusion 4-1. Hybridoma fusion Project report 1-2 weeks 5. Screening and subcloning 5-1. Positive clones screening 500µL supernatant per clone, about 10 clones
Project report
Hybridoma clone (1 vial of each clone)3-4 weeks 5-2. 2 rounds of subcloning 6. Antibody production & purification - Optional 6-1. Hybridoma culture Antibody sample (3-10mg/clone, liquid)
Hybridoma cells (2 vials of each clone)3-4 weeks 6-2. Inject the hybridoma cells into mice to prepare ascites 6-3. Purify the antibody from the ascites 四、客戶提供資料
1. 抗原資訊(名稱、序列)
2. 是否需要代為生產抗原
3. 後續應用
4. 抗體純化方式(Protein A/G或Antigen affinity purification)
1. 結案報告
五、到貨規格
2. 500uL克隆上清液
3. 融合瘤細胞株(1~2株)
4. 100ug蛋白(如有委託生產抗原)
5. 3-10mg抗體(如有委託純化抗體)
Mouse anti-SETD3 mAb published in Cell Death Dis. 2019;10(3):183. Mouse anti-P. gingivalis mAb published in ONCOLOGY LETTERS 21: 189, 2021.
